Tissue Culture explained

caliloo(z6/7)January 1, 2013

Tissue Culture Explained

Set the picture in your mind ... think of a single eye of a hosta as an artichoke ....

There is a bud under every junction of the leaf at the crown .... the first ones are visible to the naked eye ... as they are now swelling for next year .... as you remove more leaves, they get smaller and smaller ... these are dormant buds ... they may never become undormant ... or should i say dominant ...

A TC'er will take a bud .... strip it of all leaves ... sterilize the bee-gee-bees out of it and the work station... and put it in a beaker with food and hormones ... at this stage the hormones will tell the bud to unlock the dormant buds ....

They will come back after a given period of time ... and there will be all these little ex-plants on the bud .... maybe the a sixteenth by 1/8th inch ....

They will then take these off and put them in a new beaker with a new hormone to cause root growth .... if there was too much budding hormone .... the plant can continue to force dormant buds all thru the growth cycle ... the balance on all these hormones is very delicate ...

They put them under the lights for another time period ... and then move them up to cell packs ...

Some tc'ers sell them now as tiny, tiny runts ... there is barely anyway to tell if the plant is true to name .... as they are barely bigger than your thumb at this time ....

Other tc'ers will grow them up thru 2x4 inch cell packs ... these tc'ers provide the customer a 6 inch plant with multiple leaves ... and we can be pretty sure they are true to name ....

Contributed by ken adrian

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